Search on: POLYMERASE CHAIN REACTION 
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Descriptor English:   Polymerase Chain Reaction 
Descriptor Spanish:   Reacción en Cadena de la Polimerasa 
Descriptor Portuguese:   Reação em Cadeia da Polimerase 
Synonyms English:   Anchored PCR
Anchored Polymerase Chain Reaction
Inverse PCR
Inverse Polymerase Chain Reaction
Nested PCR
Nested Polymerase Chain Reaction
PCR
PCR, Anchored
PCR, Inverse
PCR, Nested
Polymerase Chain Reactions
Reaction, Polymerase Chain
Reactions, Polymerase Chain  
Tree Number:   E05.393.620.500
Definition English:   In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. 
See Related English:   Ligase Chain Reaction
Sequence Tagged Sites
 
History Note English:   91(90); was see under GENE AMPLIFICATION 1990 
Allowable Qualifiers English:  
CL classification EC economics
ES ethics HI history
IS instrumentation MT methods
ST standards SN statistics & numerical data
TD trends VE veterinary
Record Number:   28320 
Unique Identifier:   D016133 

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