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Descriptor English: Oligonucleotide Probes
Descriptor Spanish: Sondas de Oligonucleótidos
Descriptor sondas de oligonucleótidos
Entry term(s) sonda de oligonucleótidos
sondas de oligodesoxirribonucleótidos
sondas de oligorribonucleótidos
Scope note: Oligonucleótidos sintéticos o naturales que se utilizan en estudios de hibridación con el propósito de identificar y estudiar fragmentos específicos de ácidos nucleicos, por ejemplo, segmentos de ADN que se encuentran cerca o dentro de locus de genes específicos o de genes. La sonda se hibrida con un ARNm específico, de estar presente. Entre las técnicas convencionales que se usan para evaluar el producto de la hibridación se encuentran la transferencia puntual (dot blot), la transferencia de Southern (Southern blot) y las pruebas de anticuerpos específicos de híbridos de ADN-ARN. Las sondas suelen marcarse con los radioisótopos 32P y 125I, y con el marcador químico biotina.
Descriptor Portuguese: Sondas de Oligonucleotídeos
Descriptor French: Sondes oligonucléotidiques
Entry term(s): Oligodeoxyribonucleotide Probes
Oligonucleotide Probe
Oligoribonucleotide Probes
Probe, Oligonucleotide
Probes, Oligodeoxyribonucleotide
Probes, Oligonucleotide
Probes, Oligoribonucleotide
Tree number(s): D13.444.600.601
D27.505.259.750.600.650
D27.720.470.530.600.650
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D015345
Scope note: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Allowable Qualifiers: AD administration & dosage
AE adverse effects
AN analysis
BI biosynthesis
BL blood
CF cerebrospinal fluid
CH chemistry
CL classification
CS chemical synthesis
DE drug effects
EC economics
GE genetics
HI history
IM immunology
IP isolation & purification
ME metabolism
PD pharmacology
PH physiology
PK pharmacokinetics
PO poisoning
RE radiation effects
SD supply & distribution
ST standards
TO toxicity
TU therapeutic use
UL ultrastructure
UR urine
Previous Indexing: Nucleic Acid Hybridization (1972-1988)
Oligonucleotides (1972-1988)
Public MeSH Note: 89
History Note: 89
Related: Nucleic Acid Hybridization MeSH
Oligonucleotide Array Sequence Analysis MeSH
DeCS ID: 23787
Unique ID: D015345
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1989/01/01
Date of Entry: 1988/06/01
Revision Date: 2020/06/17
Oligonucleotide Probes - Preferred
Concept UI M0023630
Scope note Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Preferred term Oligonucleotide Probes
Entry term(s) Oligonucleotide Probe
Probe, Oligonucleotide
Probes, Oligonucleotide
Oligodeoxyribonucleotide Probes - Narrower
Concept UI M0023629
Preferred term Oligodeoxyribonucleotide Probes
Entry term(s) Probes, Oligodeoxyribonucleotide
Oligoribonucleotide Probes - Narrower
Concept UI M0023631
Preferred term Oligoribonucleotide Probes
Entry term(s) Probes, Oligoribonucleotide



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