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Descritor em português: | Imunoprecipitação da Cromatina | ||||
Descritor em inglês: | Chromatin Immunoprecipitation | ||||
Descritor em espanhol: |
Inmunoprecipitación de Cromatina
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Descritor em francês: | Immunoprécipitation de la chromatine | ||||
Termo(s) alternativo(s): |
Immunoprecipitation, Chromatin |
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Código(s) hierárquico(s): |
E05.393.170 E05.478.605.160 |
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Identificador Único RDF: | https://id.nlm.nih.gov/mesh/D047369 | ||||
Nota de escopo: | A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. |
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Qualificadores permitidos: |
AE adverse effects CL classification EC economics ES ethics HI history IS instrumentation MO mortality MT methods NU nursing PX psychology SN statistics & numerical data ST standards TD trends VE veterinary |
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Indexação Anterior: |
Precipitin Tests (2000-2004) |
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Nota MeSH pública: | 2005 |
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Nota histórica: | 2005 |
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Identificador DeCS: | 38826 | ||||
ID do descritor: | D047369 | ||||
Documentos indexados na Biblioteca Virtual em Saúde (BVS): | Clique aqui para acessar os documentos da BVS | ||||
Data de estabelecimento: | 01/01/2005 | ||||
Data de entrada: | 19/07/2004 |
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ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT
Investigative Techniques [E05]Investigative Techniques -
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT
Investigative Techniques [E05]Investigative Techniques
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Chromatin Immunoprecipitation
- Conceito preferido
Identificador do conceito |
M0457418 |
Nota de escopo | A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. |
Termo preferido | Chromatin Immunoprecipitation |
Termo(s) alternativo(s) |
Immunoprecipitation, Chromatin |
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