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Descriptor English: Enzyme-Linked Immunosorbent Assay
Descriptor Spanish: Ensayo de Inmunoadsorción Enzimática
Descriptor enzimoinmunoanálisis por adsorción
Entry term(s) EIA
ELISA
Scope note: Inmmunoanálisis que utiliza un anticuerpo marcado con una enzima marcadora como la peroxidasa del rábano picante (horseradish peroxidase). Mientras la enzima o el anticuerpo permanecen unidas a un sustrato inmunoadsorbente, ambas retienen su actividad biológica; el cambio en la actividad enzimática como resultado de la reacción enzima-anticuerpo-antígeno es proporcional a la concentración del antígeno y puede ser medida espectrofotométrica o visualmente. Se han desarrollado muchas variantes del método.
Descriptor Portuguese: Ensaio de Imunoadsorção Enzimática
Descriptor French: Test ELISA
Entry term(s): Assay, Enzyme-Linked Immunosorbent
Assays, Enzyme-Linked Immunosorbent
ELISA
Enzyme Linked Immunosorbent Assay
Enzyme-Linked Immunosorbent Assays
Immunosorbent Assay, Enzyme-Linked
Immunosorbent Assays, Enzyme-Linked
Tree number(s): E05.478.566.350.170
E05.478.566.380.360
E05.478.583.400.170
E05.601.470.350.170
E05.601.470.380.360
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D004797
Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Annotation: NIM; IMMUNOSORBENTS is also available
Allowable Qualifiers: AE adverse effects
CL classification
EC economics
ES ethics
HI history
IS instrumentation
MO mortality
MT methods
NU nursing
PX psychology
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Immunoassay (1966-1976)
Serodiagnosis (1966-1976)
Public MeSH Note: 86; was see under IMMUNOENZYME TECHNIQUES 1977-85
History Note: 86(77); was see under IMMUNOENZYME TECHNIQUES 1977-85
DeCS ID: 22443
Unique ID: D004797
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1986/01/01
Date of Entry: 1976/04/19
Revision Date: 2015/06/22
Enzyme-Linked Immunosorbent Assay - Preferred
Concept UI M0007526
Scope note An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Preferred term Enzyme-Linked Immunosorbent Assay
Entry term(s) Assay, Enzyme-Linked Immunosorbent
Assays, Enzyme-Linked Immunosorbent
ELISA
Enzyme Linked Immunosorbent Assay
Enzyme-Linked Immunosorbent Assays
Immunosorbent Assay, Enzyme-Linked
Immunosorbent Assays, Enzyme-Linked



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