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Descriptor English: Microspectrophotometry
Descriptor Spanish: Microespectrofotometría
Descriptor microespectrofotometría
Scope note: Técnica analítica para estudiar sustancias presentes en concentraciones de coenzimas en células individuales, in situ, mediante la medición de absorción de la luz. La luz de una lámpara de tungsteno o de un arco de xenón dispersada por una retícula monocromática, ilumina el sistema óptico de un microscopio. La absorción de luz se mide (en nanómetros) comparando la diferencia entre la imagen de la muestra y una imagen de referencia.
Descriptor Portuguese: Microespectrofotometria
Descriptor French: Microspectrophotométrie
Entry term(s): Microspectrophotometries
Tree number(s): E05.196.712.726.300
E05.196.867.826.300
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D015591
Scope note: Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.
Annotation: a type of spectrum anal; NIM
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Spectrophotometry (1966-1989)
Public MeSH Note: 90
History Note: 90
DeCS ID: 24782
Unique ID: D015591
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1990/01/01
Date of Entry: 1989/04/07
Revision Date: 2002/07/03
Microspectrophotometry - Preferred
Concept UI M0023935
Scope note Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.
Preferred term Microspectrophotometry
Entry term(s) Microspectrophotometries



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