ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT Investigative Techniques [E05] Investigative Techniques Genetic Techniques [E05.393] Genetic Techniques Cloning, Molecular [E05.393.220] Cloning, Molecular Artificial Gene Fusion [E05.393.220.250] Artificial Gene Fusion Two-Hybrid System Techniques [E05.393.220.870] Two-Hybrid System Techniques ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT Investigative Techniques [E05] Investigative Techniques Molecular Probe Techniques [E05.601] Molecular Probe Techniques Protein Interaction Mapping [E05.601.690] Protein Interaction Mapping Blotting, Far-Western [E05.601.690.149] Blotting, Far-Western Epitope Mapping [E05.601.690.300] Epitope Mapping Two-Hybrid System Techniques [E05.601.690.650] Two-Hybrid System Techniques ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT Investigative Techniques [E05] Investigative Techniques Molecular Probe Techniques [E05.601] Molecular Probe Techniques Biosensing Techniques [E05.601.043] Biosensing Techniques Biotinylation [E05.601.085] Biotinylation Blotting, Northern [E05.601.100] Blotting, Northern Blotting, Southern [E05.601.150] Blotting, Southern Blotting, Southwestern [E05.601.225] Blotting, Southwestern Blotting, Western [E05.601.262] Blotting, Western Branched DNA Signal Amplification Assay [E05.601.300] Branched DNA Signal Amplification Assay Immunoassay [E05.601.470] Immunoassay Molecular Imaging [E05.601.555] Molecular Imaging Oligonucleotide Array Sequence Analysis [E05.601.640] Oligonucleotide Array Sequence Analysis Protein Array Analysis [E05.601.680] Protein Array Analysis Protein Interaction Mapping [E05.601.690] Protein Interaction Mapping Random Amplified Polymorphic DNA Technique [E05.601.700] Random Amplified Polymorphic DNA Technique Two-Hybrid System Techniques [E05.601.870] Two-Hybrid System Techniques

Two-Hybrid System Techniques - Preferred

Concept UI	M0328080
Scope note	Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Preferred term	Two-Hybrid System Techniques
Entry term(s)	Assay, Two-Hybrid Assays, Two-Hybrid Method, Two-Hybrid Methods, Two-Hybrid Technic, Two-Hybrid System Technics, Two-Hybrid System Technique, Two-Hybrid System Techniques, Two-Hybrid System Two Hybrid Assay Two Hybrid Method Two Hybrid System Technics Two Hybrid System Techniques Two-Hybrid Assay Two-Hybrid Assays Two-Hybrid Method Two-Hybrid Methods Two-Hybrid System Technic Two-Hybrid System Technics Two-Hybrid System Technique

One-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0331623
Scope note	Variations on the two-hybrid system technique that analyze for proteins interacting with specific DNA sequences.
Preferred term	One-Hybrid System Techniques
Entry term(s)	One Hybrid System Technics One Hybrid System Techniques One-Hybrid System Technic One-Hybrid System Technics One-Hybrid System Technique Technic, One-Hybrid System Technics, One-Hybrid System Technique, One-Hybrid System Techniques, One-Hybrid System

Reverse One-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0331625
Scope note	Variations on the two-hybrid system technique that analyze for mutations or small molecules that interfere with or dissociate specific protein-DNA interactions.
Preferred term	Reverse One-Hybrid System Techniques
Entry term(s)	Reverse One Hybrid System Techniques

Yeast Two-Hybrid System Techniques - Narrower

Concept UI	M0503918
Preferred term	Yeast Two-Hybrid System Techniques
Entry term(s)	Assay, Yeast Two-Hybrid Assays, Yeast Two-Hybrid System, Yeast Two-Hybrid Systems, Yeast Two-Hybrid Two-Hybrid Assay, Yeast Two-Hybrid Assays, Yeast Two-Hybrid System, Yeast Two-Hybrid Systems, Yeast Yeast Two Hybrid Assay Yeast Two Hybrid System Yeast Two Hybrid System Techniques Yeast Two-Hybrid Assay Yeast Two-Hybrid Assays Yeast Two-Hybrid System Yeast Two-Hybrid Systems

Reverse Two-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0331624
Scope note	Variations on the two-hybrid system technique that analyze for mutations or small molecules that interfere with or dissociate specific protein-protein interactions.
Preferred term	Reverse Two-Hybrid System Techniques
Entry term(s)	Reverse Two Hybrid System Techniques

Three-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0331626
Scope note	Variations on the two-hybrid system technique that analyze for proteins that interact with specific RNA molecules.
Preferred term	Three-Hybrid System Techniques
Entry term(s)	Technic, Three-Hybrid System Technics, Three-Hybrid System Technique, Three-Hybrid System Technique, Tri-Hybrid System Techniques, Three-Hybrid System Techniques, Tri-Hybrid System Three Hybrid System Technics Three Hybrid System Techniques Three-Hybrid System Technic Three-Hybrid System Technics Three-Hybrid System Technique Tri Hybrid System Techniques Tri-Hybrid System Technique Tri-Hybrid System Techniques

Yeast One-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0503920
Preferred term	Yeast One-Hybrid System Techniques
Entry term(s)	Yeast One Hybrid System Techniques

Yeast Three-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0503919
Preferred term	Yeast Three-Hybrid System Techniques
Entry term(s)	Assay, Yeast Three-Hybrid Assays, Yeast Three-Hybrid System, Yeast Three-Hybrid Systems, Yeast Three-Hybrid Three-Hybrid Assay, Yeast Three-Hybrid Assays, Yeast Three-Hybrid System, Yeast Three-Hybrid Systems, Yeast Yeast Three Hybrid Assay Yeast Three Hybrid System Yeast Three Hybrid System Techniques Yeast Three-Hybrid Assay Yeast Three-Hybrid Assays Yeast Three-Hybrid System Yeast Three-Hybrid Systems

n-Hybrid System Techniques - Broader

Concept UI	M0505475
Scope note	Variations on the two-hybrid system technique.
Preferred term	n-Hybrid System Techniques
Entry term(s)	System Technique, n-Hybrid System Techniques, n-Hybrid Technique, n-Hybrid System Techniques, n-Hybrid System n Hybrid System Techniques n-Hybrid System Technique

Reverse Three-Hybrid System Techniques - Related but not broader or narrower

Concept UI	M0505544
Scope note	Variations on the two-hybrid system technique that analyze for mutations or small molecules that interfere with or dissociate specific interactions between protein and RNA molecules or other ligands.
Preferred term	Reverse Three-Hybrid System Techniques
Entry term(s)	Reverse Three Hybrid System Techniques