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Descriptor English: Freeze Etching
Descriptor Spanish: Grabado por Congelación
Descriptor criograbado
Scope note: Técnica de réplica en que las células se congelan a una temperatura muy baja y se rebanan con una cuchilla para exponer las superficies interiores de las células o de las membranas celulares. Las superficies celulares rebanadas entonces se liofilizan para exponer sus constituyentes. Las superficies están listas ahora para ser sombreadas y observadas empleando un microscopio electrónico. Este método difiere del de criofractura en que no se emplea ningún crioprotector y, por tanto, permite la sublimación del agua durante el proceso de liofilización para el grabado de las superficies.
Descriptor Portuguese: Técnica de Congelamento e Réplica
Descriptor French: Cryodécapage
Entry term(s): Etching, Freeze
Tree number(s): E01.370.225.500.620.620.260.400
E01.370.225.750.600.620.260.400
E05.200.500.620.620.260.400
E05.200.750.600.620.260.400
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D005613
Scope note: A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Annotation: do not confuse with FREEZE FRACTURING (wherein a cryoprotectant is used)
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Freezing (1966-1968)
Histological Techniques (1966-1968)
Microscopy, Electron (1966-1968)
Public MeSH Note: 73
History Note: 73(69)
DeCS ID: 5749
Unique ID: D005613
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1973/01/01
Date of Entry: 1999/01/01
Revision Date: 2011/06/24
Freeze Etching - Preferred
Concept UI M0008835
Scope note A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Preferred term Freeze Etching
Entry term(s) Etching, Freeze



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