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Descriptor English: Freeze Fracturing
Descriptor Spanish: Técnica de Fractura por Congelación
Descriptor criofractura
Entry term(s) fraccionamiento por congelación
Scope note: Preparación para microscopía electrónica de réplicas diminutas de superficies expuestas de la célula que han sido fracturadas en estado de congelación. La muestra es congelada y entonces cortada bajo un alto vacío a la misma temperatura. La superficie expuesta se sombrea con carbono y platino y se reviste con carbono para obtener una réplica de carbono.
Descriptor Portuguese: Técnica de Fratura por Congelamento
Descriptor French: Cryofracture
Entry term(s): Fracturing, Freeze
Fracturings, Freeze
Freeze Fracturings
Tree number(s): E01.370.225.500.620.620.260
E01.370.225.750.600.620.260
E05.200.500.620.620.260
E05.200.750.600.620.260
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D005614
Scope note: Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Annotation: do not confuse with FREEZE ETCHING (wherein no cryoprotectant is used)
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Freeze Etching (1969-1974)
Public MeSH Note: 75
History Note: 75
DeCS ID: 5750
Unique ID: D005614
NLM Classification: QY 95
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1975/09/01
Date of Entry: 1974/11/11
Revision Date: 2011/06/24
Freeze Fracturing - Preferred
Concept UI M0008836
Scope note Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Preferred term Freeze Fracturing
Entry term(s) Fracturing, Freeze
Fracturings, Freeze
Freeze Fracturings



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