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Descriptor English: Polymerase Chain Reaction
Descriptor Spanish: Reacción en Cadena de la Polimerasa
Descriptor reacción en cadena de la polimerasa
Entry term(s) PCR
PCR anclado
PCR anidado
PCR inverso
RCP anclado
RCP anidado
RCP inverso
Scope note: Método in vitro para producir grandes cantidades de fragmentos específicos de ADN o ARN de longitud y secuencia definidas a partir de pequeñas cantidades de cortas secuencias flanqueadoras de oligonucleótidos (cebadores). Los pasos esenciales incluyen desnaturalización térmica de las moléculas diana de doble cadena, reasociación de los cebadores, iniciadores, con sus secuencias complementarias, y extensión de los cebadores reasociados mediante síntesis enzimática con la ADN polimerasa. La reacción es eficiente, específica y extremadamente sensible. Entre los usos de la reacción está el diagnóstico de enfermedades, detección de patógenos difíciles de aislar, análisis de mutaciones, pruebas genéticas, secuenciación del ADN y el análisis de relaciones evolutivas.
Descriptor Portuguese: Reação em Cadeia da Polimerase
Descriptor French: Réaction de polymérisation en chaîne
Entry term(s): Anchored PCR
Anchored Polymerase Chain Reaction
Inverse PCR
Inverse Polymerase Chain Reaction
Nested PCR
Nested Polymerase Chain Reaction
PCR
PCR, Anchored
PCR, Inverse
PCR, Nested
Polymerase Chain Reactions
Reaction, Polymerase Chain
Reactions, Polymerase Chain
Tree number(s): E05.393.620.500
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D016133
Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Gene Amplification (1988-1989)
Public MeSH Note: 91; was see under GENE AMPLIFICATION 1990
History Note: 91(90); was see under GENE AMPLIFICATION 1990
Related: Ligase Chain Reaction MeSH
Sequence Tagged Sites MeSH
DeCS ID: 28320
Unique ID: D016133
NLM Classification: QU 550.5.P6
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1991/01/01
Date of Entry: 1990/01/11
Revision Date: 2011/06/24
Polymerase Chain Reaction - Preferred
Concept UI M0024634
Scope note In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Preferred term Polymerase Chain Reaction
Entry term(s) PCR
Polymerase Chain Reactions
Reaction, Polymerase Chain
Reactions, Polymerase Chain
Inverse PCR - Narrower
Concept UI M0024636
Preferred term Inverse PCR
Entry term(s) Inverse Polymerase Chain Reaction
PCR, Inverse
Nested Polymerase Chain Reaction - Narrower
Concept UI M0024637
Preferred term Nested Polymerase Chain Reaction
Entry term(s) Nested PCR
PCR, Nested
Anchored PCR - Narrower
Concept UI M0024635
Preferred term Anchored PCR
Entry term(s) Anchored Polymerase Chain Reaction
PCR, Anchored



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