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Descriptor English: Ligase Chain Reaction
Descriptor Spanish: Reacción en Cadena de la Ligasa
Descriptor reacción en cadena de la ligasa
Scope note: Técnica de amplificación del ADN basada en el ligamiento de SONDAS DE OLIGONUCLEOTIDOS. Las sondas son diseñadas para el exacto emparejamiento de dos secuencias adyacentes de un ADN diana específico. La reacción en cadena es repetida en tres pasos en presencia de exceso de sonda: (1) desnaturalización por el calor del ADN bicatenario, (2) hibridación de las sondas con el ADN diana, y (3) unión de las sondas por la ADN ligasa termoestable. Después de repetir la reacción 20-30 ciclos se mide la producción de sonda ligada.
Descriptor Portuguese: Reação em Cadeia da Ligase
Descriptor French: Réaction en chaîne par ligase
Entry term(s): Chain Reaction, Ligase
Chain Reactions, Ligase
Ligase Chain Reactions
Reaction, Ligase Chain
Reactions, Ligase Chain
Tree number(s): E05.393.620.311
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D022521
Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Gene Amplification (1980-2000)
Public MeSH Note: 2001
History Note: 2001
Related: Polymerase Chain Reaction MeSH
DeCS ID: 35409
Unique ID: D022521
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 2001/01/01
Date of Entry: 2000/06/22
Ligase Chain Reaction - Preferred
Concept UI M0358403
Scope note A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Preferred term Ligase Chain Reaction
Entry term(s) Chain Reaction, Ligase
Chain Reactions, Ligase
Ligase Chain Reactions
Reaction, Ligase Chain
Reactions, Ligase Chain



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