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Descriptor English: Negative Staining
Descriptor Spanish: Coloración Negativa
Descriptor tinción negativa
Scope note: Técnica que lava las muestras de tejidos con una solución concentrada de una sal de metal pesado, dejándola secar. La muestra se recubre con una capa muy fina de la sal metálica, excluyéndose las áreas donde está presente una macromolécula adsorbida. Las macromoléculas permiten que los electrones del rayo de un microscopio electrónico pasen con mucha más facilidad que el metal pesado, obteniéndose así una imagen inversa o negativa de la molécula.
Descriptor Portuguese: Coloração Negativa
Descriptor French: Coloration négative
Entry term(s): Staining, Negative
Tree number(s): E01.370.225.500.620.670.520
E01.370.225.750.600.670.520
E05.200.500.620.670.520
E05.200.750.600.670.520
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D016624
Scope note: The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Staining (1966-1991)
Public MeSH Note: 92
History Note: 92
DeCS ID: 29939
Unique ID: D016624
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1992/01/01
Date of Entry: 1991/01/25
Revision Date: 2011/06/24
Negative Staining - Preferred
Concept UI M0025341
Scope note The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Preferred term Negative Staining
Entry term(s) Staining, Negative



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