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Descriptor English: Flow Cytometry
Descriptor Spanish: Citometría de Flujo
Descriptor citometría de flujo
Entry term(s) citofluorometría de flujo
microfluorometría de flujo
Scope note: Técnica que emplea un sistema instrumental para realizar, procesar y exhibir una o más mediciones de células individuales obtenidas de una suspensión celular. Las células generalmente se tiñen con uno o más colorantes fluorescentes específicos para los componentes celulares de interés, por ejemplo, el ADN, y la fluorescencia de cada célula se mide cuando atraviesa rápidamente el haz de excitación (láser o lámpara de arco de mercurio). La fluorescencia brinda una medición cuantitativa de diversas propiedades bioquímicas y biofísicas de la célula como base para diferenciación celular. Otros parámetros ópticos mensurables incluyen la absorción y la difusión de la luz, aplicándose esta última a la medición del tamaño, forma, densidad, granularidad de la célula y su absorción del colorante.
Descriptor Portuguese: Citometria de Fluxo
Descriptor French: Cytométrie en flux
Entry term(s): Cell Sorting, Fluorescence-Activated
Cell Sortings, Fluorescence-Activated
Cytofluorometries, Flow
Cytofluorometry, Flow
Cytometries, Flow
Cytometry, Flow
Flow Cytofluorometries
Flow Cytofluorometry
Flow Cytometries
Flow Microfluorimetry
Flow Microfluorometries
Flow Microfluorometry
Fluorescence Activated Cell Sorting
Fluorescence-Activated Cell Sorting
Fluorescence-Activated Cell Sortings
Microfluorimetry, Flow
Microfluorometries, Flow
Microfluorometry, Flow
Sorting, Fluorescence-Activated Cell
Sortings, Fluorescence-Activated Cell
Tree number(s): E01.370.225.500.363.342
E01.370.225.500.386.350
E05.196.712.516.600.240.350
E05.200.500.363.342
E05.200.500.386.350
E05.242.363.342
E05.242.386.350
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D005434
Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Annotation: flow cell sorting & FLUORESCENCE-ACTIVATED CELL SORTING: coord with CELL SEPARATION
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Public MeSH Note: 82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90
Online Note: use FLUOROIMMUNOASSAY to search FLOW MICROFLUORIMETRY 1989-90
History Note: 82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90
DeCS ID: 5567
Unique ID: D005434
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1982/01/01
Date of Entry: 1981/02/24
Revision Date: 2015/06/01
Flow Cytometry - Preferred
Concept UI M0008573
Scope note Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Preferred term Flow Cytometry
Entry term(s) Cytofluorometries, Flow
Cytofluorometry, Flow
Cytometries, Flow
Cytometry, Flow
Flow Cytofluorometries
Flow Cytofluorometry
Flow Cytometries
Flow Microfluorimetry
Flow Microfluorometries
Flow Microfluorometry
Microfluorimetry, Flow
Microfluorometries, Flow
Microfluorometry, Flow
Fluorescence-Activated Cell Sorting - Narrower
Concept UI M0008574
Preferred term Fluorescence-Activated Cell Sorting
Entry term(s) Cell Sorting, Fluorescence-Activated
Cell Sortings, Fluorescence-Activated
Fluorescence Activated Cell Sorting
Fluorescence-Activated Cell Sortings
Sorting, Fluorescence-Activated Cell
Sortings, Fluorescence-Activated Cell



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