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Descriptor English: DNA Probes
Descriptor Spanish: Sondas de ADN
Descriptor sondas de ADN
Entry term(s) sonda de ADN
sonda de hibridación de ADN
sondas cromosómicas
sondas genéticas de ADN
Scope note: ADN específico de especies o subespecies (incluidos el ADN COMPLEMENTARIO, genes conservados, cromosomas completos o todo el genoma) que se utiliza en estudios de hibridación con el fin de identificar microorganismos, medir homologías ADN-ADN, agrupar subespecies, etcétera. La sonda de ADN se hibrida con un ARNm específico, de estar presente éste. Entre las técnicas convencionales que se utilizan para determinar el producto de hibridación se encuentran los ensayos de inmunotransferencia por puntos ("dot blot"), de transferencia de Southern ("Southern-blot") y pruebas de anticuerpos específicos de híbridos ADN:ARN. Entre los marcadores convencionales de las sondas de ADN se encuentran los marcadores radioactivos 32P y 125I y el marcador químico biotina. El empleo de sondas de ADN proporciona un sustituto específico, sensible, rápido y barato de las técnicas de cultivo celular para el diagnóstico de las infecciones.
Descriptor Portuguese: Sondas de DNA
Descriptor French: Sondes d'ADN
Entry term(s): Chromosomal Probes
Chromosomal Probes, Whole
Conserved Gene Probes
DNA Gene Probes
DNA Hybridization Probe
DNA Hybridization Probes
DNA Probe
Gene Probes, Conserved
Gene Probes, DNA
Hybridization Probe, DNA
Hybridization Probes, DNA
Probe, DNA
Probe, DNA Hybridization
Probes, Chromosomal
Probes, Conserved Gene
Probes, DNA
Probes, DNA Gene
Probes, DNA Hybridization
Probes, Whole Chromosomal
Whole Chromosomal Probes
Whole Genomic DNA Probes
Tree number(s): D13.444.600.223
D27.505.259.750.600.223
D27.720.470.530.600.223
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D015342
Scope note: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Annotation: general or unspecified; prefer specifics
Allowable Qualifiers: AD administration & dosage
AE adverse effects
AN analysis
BI biosynthesis
BL blood
CF cerebrospinal fluid
CH chemistry
CL classification
CS chemical synthesis
DE drug effects
EC economics
GE genetics
HI history
IM immunology
IP isolation & purification
ME metabolism
PD pharmacology
PH physiology
PK pharmacokinetics
PO poisoning
RE radiation effects
SD supply & distribution
ST standards
TO toxicity
TU therapeutic use
UL ultrastructure
UR urine
Previous Indexing: DNA or specific DNA (1972-1988)
Nucleic Acid Hybridization (1972-1988)
Public MeSH Note: 89
History Note: 89
Related: Chromosome Walking MeSH
DeCS ID: 23616
Unique ID: D015342
NLM Classification: QU 58.5
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1989/01/01
Date of Entry: 1988/06/01
Revision Date: 2020/05/27
DNA Probes - Preferred
Concept UI M0023617
Scope note Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Preferred term DNA Probes
Entry term(s) DNA Hybridization Probe
DNA Hybridization Probes
DNA Probe
Hybridization Probe, DNA
Hybridization Probes, DNA
Probe, DNA
Probe, DNA Hybridization
Probes, DNA
Probes, DNA Hybridization
Whole Genomic DNA Probes - Narrower
Concept UI M0023620
Preferred term Whole Genomic DNA Probes
Gene Probes, DNA - Narrower
Concept UI M0023618
Preferred term Gene Probes, DNA
Entry term(s) DNA Gene Probes
Probes, DNA Gene
Whole Chromosomal Probes - Narrower
Concept UI M0023619
Preferred term Whole Chromosomal Probes
Entry term(s) Chromosomal Probes, Whole
Probes, Whole Chromosomal
Chromosomal Probes - Narrower
Concept UI M0023615
Preferred term Chromosomal Probes
Entry term(s) Probes, Chromosomal
Conserved Gene Probes - Narrower
Concept UI M0023616
Preferred term Conserved Gene Probes
Entry term(s) Gene Probes, Conserved
Probes, Conserved Gene



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