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Descriptor English: Heteroduplex Analysis
Descriptor Spanish: Análisis Heterodúplex
Descriptor análisis heterodúplex
Scope note: Método para detectar mutaciones genéticas mezclando ADN de tipo salvaje y un mutante amplificado por PCR, seguido de desnaturalización y apareamiento. Los productos resultantes se separan mediante electroforesis en gel, con sustituciones de una única base detectables bajo condiciones electroforéticas y formulaciones de gel óptimas. También se pueden analizar defectos de acoplamiento de pares de bases grandes empleando el microscopio electrónico para visualizar las regiones heterodúplex.
Descriptor Portuguese: Análise Heteroduplex
Descriptor French: Analyse d'hétéroduplex
Entry term(s): Analyses, Heteroduplex
Analysis, Heteroduplex
Heteroduplex Analyses
Tree number(s): E05.393.661.250
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D020180
Scope note: A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Nucleic Acid Heteroduplexes (1980-1998)
Nucleic Acid Hybridization (1973-1979)
Public MeSH Note: 99
History Note: 99
Entry Version: HETERODUPLEX ANAL
Related: Base Pair Mismatch MeSH
Nucleic Acid Heteroduplexes MeSH
DeCS ID: 33874
Unique ID: D020180
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1999/01/01
Date of Entry: 1998/06/10
Heteroduplex Analysis - Preferred
Concept UI M0029895
Scope note A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.
Preferred term Heteroduplex Analysis
Entry term(s) Analyses, Heteroduplex
Analysis, Heteroduplex
Heteroduplex Analyses



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