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Descriptor English: In Situ Nick-End Labeling
Descriptor Spanish: Etiquetado Corte-Fin in Situ
Descriptor etiquetado in situ de extremos de corte de ADN
Entry term(s) TUNEL (Terminal Transferase-Mediated dUTP-Biotin Nick End Labeling)
etiquetado in situ del extremo de corte
marcado in situ del extremo libre por dUTP
técnica de TUNEL
Scope note: Método in situ para detectar áreas del ADN fragmentadas durante la apoptosis. Se usa desoxinucleotidil transferasa terminal para agregar dUTP marcado, sin cadena plantilla, a los extremos OH 3' terminales del ADN de cadena simple o doble. El ensayo de corte y marcado de la desoxinucleotidil transferasa, o TUNEL, marca la apoptosis de células individuales y es más sensible que la electroforesis con gel de agarosa para analizar la fragmentación del ADN.
Descriptor Portuguese: Marcação In Situ das Extremidades Cortadas
Descriptor French: Méthode TUNEL
Entry term(s): In Situ Nick End Labeling
In Situ Nick End Labelling
In Situ Nick End-Labeling
In Situ Nick End-Labelling
In Situ Nick-End Labelling
Nick End Labeling, In Situ
Nick End Labelling, In Situ
Nick End-Labeling, In Situ
Nick End-Labelling, In Situ
Nick-End Labeling, In Situ
Nick-End Labelling, In Situ
TUNEL
Tree number(s): E05.393.475
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D020287
Scope note: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
Allowable Qualifiers: CL classification
EC economics
ES ethics
HI history
IS instrumentation
MT methods
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Apoptosis (1994-1998)
Public MeSH Note: 99
History Note: 99
Related: Apoptosis MeSH
Comet Assay MeSH
DeCS ID: 33873
Unique ID: D020287
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 1999/01/01
Date of Entry: 1998/06/10
Revision Date: 2006/07/05
In Situ Nick-End Labeling - Preferred
Concept UI M0029936
Scope note An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
Preferred term In Situ Nick-End Labeling
Entry term(s) In Situ Nick End Labeling
In Situ Nick End Labelling
In Situ Nick End-Labeling
In Situ Nick End-Labelling
In Situ Nick-End Labelling
Nick End Labeling, In Situ
Nick End Labelling, In Situ
Nick End-Labeling, In Situ
Nick End-Labelling, In Situ
Nick-End Labeling, In Situ
Nick-End Labelling, In Situ
TUNEL



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