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Descriptor English: Chromatin Immunoprecipitation
Descriptor Spanish: Inmunoprecipitación de Cromatina
Descriptor inmunoprecipitación de cromatina
Scope note: Técnica para identificar secuencias específicas de ADN que se unen in vivo a proteínas de interés. Dicha técnica requiere la fijación con formaldehído de la CROMATINA para interconectar las PROTEÍNAS DE UNIÓN AL ADN. Después de fragmentar el ADN, se aíslan complejos específicos de ADN y proteína mediante inmunoprecipitación con ANTICUERPOS dirigidos específicamente contra la proteína. A continuación se identifica el ADN aislado de tales complejos por técnicas de amplificación por PCR y secuenciación.
Descriptor Portuguese: Imunoprecipitação da Cromatina
Descriptor French: Immunoprécipitation de la chromatine
Entry term(s): Immunoprecipitation, Chromatin
Tree number(s): E05.393.170
E05.478.605.160
RDF Unique Identifier: https://id.nlm.nih.gov/mesh/D047369
Scope note: A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Allowable Qualifiers: AE adverse effects
CL classification
EC economics
ES ethics
HI history
IS instrumentation
MO mortality
MT methods
NU nursing
PX psychology
SN statistics & numerical data
ST standards
TD trends
VE veterinary
Previous Indexing: Precipitin Tests (2000-2004)
Public MeSH Note: 2005
History Note: 2005
DeCS ID: 38826
Unique ID: D047369
Documents indexed in the Virtual Health Library (VHL): Click here to access the VHL documents
Date Established: 2005/01/01
Date of Entry: 2004/07/19
Chromatin Immunoprecipitation - Preferred
Concept UI M0457418
Scope note A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Preferred term Chromatin Immunoprecipitation
Entry term(s) Immunoprecipitation, Chromatin



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