DeCS

Descriptor English:

CRISPR-Associated Protein 9

Descriptor Spanish:

Proteína 9 Asociada a CRISPR

Spanish from Spain

Descriptor	proteína 9 asociada a CRISPR
Entry term(s)	endonucleasa Cas9 enzima Cas9 proteína Cas9
Scope note:	Endodesoxirribonucleasa guiada por el ARN que se asocia a SECUENCIAS CRISPR en STREPTOCOCCUS PYOGENES y en otras bacterias en las que participa en una función de inmunidad adaptativa para escindir el ADN extraño complementario al ARN GUÍA de pequeño tamaño (ARNgp). Desde el punto de vista estructural, Cas9 consta de un módulo de HÉLICE ALFA y de un módulo de nucleasa conectados por una hélice simple. El módulo de nucleasa contiene dos dominios enzimáticos: RuvC, que escinde una cadena no diana de ADN, y un dominio de nucleasa HNH, que escinde la cadena diana. La especificidad por la diana de ADN depende de la presencia de una secuencia conocida como motivo adyacente al protoespaciador (PAM), que es una secuencia de ADN de 2-6 nucleótidos que sigue inmediatamente a la secuencia diana de Cas9.

Descriptor Portuguese:

Proteína 9 Associada à CRISPR

Descriptor French:

Protéine-9 associée à CRISPR

Entry term(s):

CRISPR Associated Protein 9
Cas9 Endonuclease
Cas9 Enzyme
Cas9 Protein
Endonuclease, Cas9
Enzyme, Cas9

Tree number(s):

D08.811.277.352.355.325.150
D12.776.097.219
D12.776.212.500

RDF Unique Identifier:

https://id.nlm.nih.gov/mesh/D000076987

Scope note:

An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9.

Allowable Qualifiers:

AD administration & dosage
AE adverse effects
AI antagonists & inhibitors
AN analysis
BI biosynthesis
BL blood
CF cerebrospinal fluid
CH chemistry
CL classification
CS chemical synthesis
DE drug effects
DF deficiency
EC economics
GE genetics
HI history
IM immunology
IP isolation & purification
ME metabolism
PD pharmacology
PH physiology
PK pharmacokinetics
PO poisoning
RE radiation effects
SD supply & distribution
ST standards
TO toxicity
TU therapeutic use
UL ultrastructure
UR urine

Concept UI	M000638955
Scope note	An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9.
Preferred term	CRISPR-Associated Protein 9
Entry term(s)	CRISPR Associated Protein 9 Cas9 Endonuclease Cas9 Enzyme Cas9 Protein Endonuclease, Cas9 Enzyme, Cas9

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